It's vital that the submerged enlargement cultures in inserts get to one hundred% confluence in advance of air-lifting. At one hundred% confluence, the cells will address the floor throughout the insert forming an entire, uniform monolayer.
Once the run is total, retrieve the Delivery Cartridge from the instrument. The cell sample should be at the bottom or facet of the collection tube. Take note: It is usually recommended to spin down the Shipping and delivery Cartridge inside a mini-centrifuge to get a couple of seconds for whole volume recovery.
Evaluate the opportunity poisonous results of candidate therapeutics, like compact molecule compounds and biologics.
It can be crucial to quench the dissociation reagent making use of FACS buffer or halt medium, by adding at the least precisely the same or double the quantity of the dissociation reagent.
This solution is designed for use in the subsequent analysis area(s) as Element of the highlighted workflow stage(s). Explore these workflows To find out more in regards to the other products we provide to assistance Each individual investigate space. Analysis Space Workflow Stages
Whilst Now we have in a roundabout way analyzed nasal epithelial cells in-house, you can find publications that report PneumaCult™ supporting this cell form.
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*This is a grasp elements record for that under protocols—all reagents pointed out in Just about every protocol are summarized.
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